fixed cell ready dapi Search Results


dapi  (Vector Laboratories)
96
Vector Laboratories dapi
Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucblue fixed cell readyprobes reagent  (Thermo Fisher)
99
Thermo Fisher nucblue fixed cell readyprobes reagent
Nucblue Fixed Cell Readyprobes Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4′,6-diamidino-2-phenylindole (dapi  (Millipore)
90
Millipore 4′,6-diamidino-2-phenylindole (dapi
4′,6 Diamidino 2 Phenylindole (Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dapi  (Beijing Solarbio Science)
90
Beijing Solarbio Science dapi
Dapi, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dapi  (Thermo Fisher)
86
Thermo Fisher dapi
Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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dapi nucblue fixed cell stain readyprobes reagent r37606  (Thermo Fisher)
90
Thermo Fisher dapi nucblue fixed cell stain readyprobes reagent r37606
Dapi Nucblue Fixed Cell Stain Readyprobes Reagent R37606, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dapi/triton x-100 solution  (Millipore)
90
Millipore dapi/triton x-100 solution
Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.
Dapi/Triton X 100 Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gold antifade reagent dapi (4′ 6-diamidino-2- phenylindole  (Thermo Fisher)
90
Thermo Fisher gold antifade reagent dapi (4′ 6-diamidino-2- phenylindole
Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.
Gold Antifade Reagent Dapi (4′ 6 Diamidino 2 Phenylindole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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prolong diamond antifade mountant with dapi  (Thermo Fisher)
86
Thermo Fisher prolong diamond antifade mountant with dapi
Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.
Prolong Diamond Antifade Mountant With Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc dapi
Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.
Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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dapi  (Beyotime)
99
Beyotime dapi
Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.
Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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nucblue fixed cell readyprobes reagent dapi  (Thermo Fisher)
86
Thermo Fisher nucblue fixed cell readyprobes reagent dapi
Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.
Nucblue Fixed Cell Readyprobes Reagent Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Image Search Results


Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.

Journal: American Journal of Cancer Research

Article Title: An unprecedented endocrine target for ovarian cancer: inhibiting 17β-HSD7 supresses cancer cell proliferation and arrests G2/M cycle

doi:

Figure Lengend Snippet: Cell proliferation and cell cycle analysis after 17β-HSD7 siRNA transfection 96 h in EOC cells. 100 nM mixed 17β-HSD7 specific siRNA and control siRNA were used. Different hormone sources were provided: E1 (0.1 nM) and DHEA (100 nm and 1 µM). A. Total RNA was extracted from OVCAR-3 cells. qRT-PCR determined the 17β-HSD7 mRNA level 72 h after siRNA transfection. Means and standard deviations are presented (n=3). B. Data reported as % of DNA synthesis vs. hormone-free control (100%). After treatment with siRNA for 96 h, 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. C. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. D. Cell cycle was analyzed by flow cytometry with DAPI/Triton X-100 solution. 17β-HSD7 siRNA was compared with control siRNA in OVCAR-3 cells. E. 17β-HSD7 siRNA was compared with control siRNA in SKOV-3 cells. Data are shown as the percentage of total cells in G0/G1, S, and G2/M phase. Quadruple wells were used for each condition and repeated in three independent experiments. Error bars represent SD. *P≤0.05 vs. control; **P≤0.001 vs. control by Student’s test.

Article Snippet: The cells were fixed with 70% ethanol and stained with DAPI/Triton X-100 solution (Sigma, St. Louis, MI, USA) before analysis by flow cytometry using the BD LSR II (BD Bioscience).

Techniques: Cell Cycle Assay, Transfection, Quantitative RT-PCR, DNA Synthesis, Flow Cytometry